Application of Hptlc Densitometric Method for Simultaneous Quantification of Rosuvastatin Calcium and Aspirin

A simple and sensitive thin-layer chromatographic method has been established for analysis of rosuvastatin calcium and aspirin in bulk & capsule dosage form. Chromatography on silica gel 60 F254 plates using toluene:butanol:methanol (9:1:0.2 v/v/v) as the mobile phase furnished compact spots at R f 0.32±0.02 and 0.67±0.03. Densitometric analysis of rosuvastatin calcium and aspirin was carried out in the absorbance mode at 234 nm. The minimum amount that could be authentically detected was found to be 22 and 156 ng for rosuvastatin calcium and aspirin respectively. Limit of Quantitation was found to be 68 and 445 ng for rosuvastatin calcium and aspirin respectively. The linear regression analysis data for the calibration plots showed good linear relationship with respect to peak area in the concentration range 200–1200 ng/spot for rosuvastatin calcium and 1500-9000 ng/spot for aspirin. The method was validated for precision, accuracy and robustness as per ICH guidelines. The proposed HPTLC method can be applied for identification and quantitative determination of rosuvastatin calcium and aspirin in bulk drug and capsule dosage form.